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Agarose bound Ricinus Communis Agglutinin II (RCA II, RCA60 , ricin)

Catalog Number: AL-1093

Availability: In stock

$170.00
Unit Size: 2 ml

Agarose bound Ricinus Communis Agglutinin II (RCA II, RCA60 , ricin) Zoom

Overview

    Features:

    • Matrix is heat stable, cross-linked 4% agarose beads with a molecular exlusion of about 2x107 daltons
    • Bead diameter ranges in size from 45-165 microns
    • Matrix is stable in solutions at pH 3-11 as well as many organic solvents
    • Immobilized lectins are prepared using affinity purified lectins
    • Covalent attachment preserves lectin activity and minimizes conformational changes that might result in nonspecific or hydrophobic interactions
    • Hydrophilic spacer arm is inserted between the lectin and the matrix
    • Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
    • No residual charges present after conjugation.  This minimizes non-specific binding to the matrix
    • Product supplied as a 1:1 suspension in buffer
    • 4 mg lectin/ml gel
    • Inhibiting/Eluting Sugar: 200 mM galactose or lactose or Glycoprotein Eluting Solution (ES-2100)

     Please note: Due to the toxicity of this product, special packaging is required and there is an additional shipping charge, sold in USA only.

    Additional Information

    Catalog Number AL-1093-2
    Unit Size 2 ml
    Country of Manufacture United States
    Applications Glycobiology, Affinity Chromatography
    Matrix Conjugate Lectins
    Sugar Specificity Galactose, N-Acetylgalactosamine, Lactose
    Conjugate Agarose
Additional Info

    Details

    Ricinus communis agglutinin II is an extremely toxic glycoprotein consisting of two disulfide-linked chains of about 28 kDa and 32 kDa, termed A and B chain, respectively. The B chain can bind to cell surfaces via galactose or N-acetylgalactosamine residues of membrane glycoconjugates and facilitates the transport of the lectin into the cell. The A chain has an enzyme activity which can catalytically block protein synthesis and is so toxic that only a single molecule of A chain is required to kill a cell. Alone, the A chain is incapable of entering the cell and is thus not toxic.

    RCA II appears to be a family of related lectins with different toxicities. Our RCA II is the most toxic lectin of this family, probably ricin D, having an I.P. L.D50 in 20 g mice of less than 50 ng.

    Agarose bound* Ricinus communis agglutinin II is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.

    This coupling method provides several advantages over the traditional cyanogen bromide procedure:

    • Maximum carbohydrate binding activity of the coupled lectins is retained
    • Linkage is stable over a range of pH values
    • Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
    • No residual charges are present after conjugation. This minimizes non-specific binding to the matrix.

    Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.

    Inhibiting/Eluting Sugar: 200 mM galactose or lactose

    Please note: Due to the toxicity of this product, special packaging is required and there is an additional shipping charge, sold in USA only.

    *4 mg lectin/ml gel

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